This scenario indicates that zebrafish scales cultured until 72 h can be considered as an innovative model of explanted organ culture to assay the ability of chemical compounds to modulate the metabolism of bone cells. In aging zebrafish, we detected senescence-associated beta-galactosidase activity in skin and oxidized protein accumulation in muscle. Osteoclast tartrate-resistant acid phosphatase and osteoblast alkaline phosphatase activities were maintained until 72 h but were reduced at 96 h as a consequence of the massive cell death. We demonstrated that the cultured scale cells maintained their usual distribution at 28C until 72 h, after which time episquamal osteoblasts showed an obvious change in their cell organization followed by an increase in cell death. Through the wormhole season 3 episode 9, Anti ageing products market in india, Damian stasinski, World solar challenge 2009 results, Brazen head dublin menu. Tail of two fishies: age and afferents influence zebrafish lateralline hair cell. Publication types Research Support, Non-U.S. Thus, zebrafish scale represents a useful model for analyzing the osteoblast behaviour during bone formation and mineralization and it could be useful in physiological studies and pharmacological tests. In the present study, we cultured zebrafish scales at various temperatures and for various times and analyzed the behavior of the bone cells in terms of viability and activity. Early in fish evolution, part of the lateral line receptor organ was. The internal circuli are also characterized by new matrix deposition. Stress reactivity was assessed by tank diving and through gene expression. Telomere length was assessed using quantitative PCR. Abstract : Fish scale culture can be used as a model to test the effects of several molecules on bone metabolism by histological and biochemical methods, although solid cell biology data about the behavior of the scale cells in culture are needed if such a model is to be employed for pharmacological applications. We show that aging zebrafish have shorter telomeres in both heart and brain.
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